Cdh5-creert2-tdtomato
WebNov 19, 2024 · The Cdh5-CreER, R26R-tdT (tdTomato), Cdh5-DreER, R26R-rox-tdT, UBC-CreER, NR1, Col1a2-CreER, ... cells in the early developing heart contribute to fibroblasts. 59 Fate mapping of ECs at the adult stage by VE-cadherin-CreERT2 showed that they contribute to the ECs but not the fibroblasts of the TAC hearts. 14 In this study, ... WebFig. 4 Detection of tdTomato (red) in various tissues of Cdh5 CreERT2/+; Rosa26 tdTomato/+ mice. CreERT2 mediated recombination can be detected in the brain, pituitary gland, eyes, heart, lung, liver, submandibular gland, kidney, pancreas, testis, muscle, intestine, colon, spleen, thymus and brown fat. TdTomato expression can not be …
Cdh5-creert2-tdtomato
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WebCdh5(PAC)-CreERT2 with well-characterized Tie2-Cre mice. Results: Adult Cdh5(PAC)-CreERT2 mice are endothelial specific but require a dose of 10 mg of tamox-ifen to allow constant Cre expression. Pdgfb-iCreERT2 mice injected with 5 mg of tamoxifen are appropriate for most endothelial research fields except liver studies, as hep- WebMay 17, 2024 · The tamoxifen-responsive conditional Cdh5-CreERT2 is commonly used for endothelial cell specific conditional deletion of loxP-flanked gene sequences. To address …
WebThe Tg(Cdh5-cre/ERT2) CIVE23Mlia allele (or VE-cadherin-CreERT2) was developed using the same 2.2 kb Cdh5 enhancer/promoter element previously used to drive the constitutively active VE-cadherin-Cre. 33,46 … WebDec 1, 2006 · Additionally, we used an inducible endothelial lineage tracing system of Ind.Endotrack (Cdh5-CreER T2 -TdTomato) mice 20, 21, in which TdTomato is constitutively expressed after Tamoxifen ...
WebPlasmid CDH5-AbR-GFP from Dr. Budd A. Tucker's lab contains the inserts CDH5p, CopGFP, and Zeocin and is published in Unpublished This plasmid is available through … WebIn inducible Pdgfb-iCreERT2;mT/mG mice injected with 5 mg tamoxifen and Cdh5(PAC)-CreERT2;mT/mG mice injected with 10 mg tamoxifen, 1 and 2 months after tamoxifen injection, all hematopoietic cells, including megakaryocytes (Fig. 4A, empty arrowheads) expressed tdTomato, suggesting that no hematopoietic expression of the Cre had …
WebMay 17, 2024 · The tamoxifen-responsive conditional Cdh5-CreERT2 is commonly used for endothelial cell specific conditional deletion of loxP …
WebSep 24, 2024 · To achieve induction of Cre gene, female Cdh5-CreERT2-TdTomato mice were gavaged with 400mg/kg of tamoxifen, followed by a two week wash out period. Extracted molecule: total RNA: Extraction protocol: Endothelial mouse lung cells were isolated as previously described (Fehrenbach et al, Am J Physiol Lung Cell Mol Physiol … structures of the mouth anatomyWebView Tg(Cdh5-cre/ERT2)1Rha allele: origin, molecular description and human disease models, images, gene associations, and references. structures of the kneeWebSep 14, 2024 · Hlf tdTomato, Runx1-creERT2 (eR1-creERT2), Cdh5-creERT2, ROSA tdTomato, ROSA YFP, Vav-icre, Tie2-cre, Evi1 +/−, Evi1 GFP/+ mice have described … structures of the outer earWebThe MMPC is a National Institutes of Health-sponsored resource that provides experimental testing services to scientists studying diabetes, obesity, diabetic complications, and other metabolic diseases in mice. structures of the human bodyWebJan 12, 2024 · a, Cdh5 CreERT2;R26 tdTomato construct of the double transgenic mice used for 3D surface rendering of adult aortae to visualize the spatial location of intimal CD45 + cells (green) relative to the ... structures of the nasopharynxWebWe utilized both a reporter mouse (B6.129 (Cg)-Gt (ROSA)26Sor tm4 (ACTB-tdTomato,-EGFP)Luo /J) and endothelin converting enzyme-1 floxed transgenic mouse line to evaluate Cre-ERT2 activity. We observed spontaneous Cre activity in both settings. Unintended Cre activity is a confounding factor that has a potentially large impact on data ... structures of the humerusWebIn Cdh5-CreERT2.Rosa26 tdTomato.CX 3 CR1 GFP mice, cells expressing the CX 3 CR1 receptor are constitutively GFP +, and treatment with 4OHT conditionally labels … structures of the nervous system practice