WebE , Flp-In 293 background cells or cells expressing NUBP2 were either directly harvested or after treatment with 1% formaldehyde. Anti-HA immunoprecipitation were performed. WCEs and anti-HA immunoprecipitates were blotted for CIAO3, CIA targeting complex components, and the CIA substrate CDKAL1. WebFlp-In™ stable cell lines are isogenic and hence protein expression is constant across a population of cells. A useful addition to the Flp-In™ system (Flp-In™ T-Rex™) allows this expression to be controlled by the addition of a small molecule inducer to the cell culture …

Flp In T Rex 293 Cell Line Hek293 Thermo Fisher Bioz

WebJul 4, 2024 · In the present study, we employed Flp-In-293 cells with the Flp-In™ system to establish cells expressing WT or non-synonymous SNP variants of human ABCC4 (Figure 1 and Table 1). Flp-In-293 cells were transfected with the ABCC4 cDNA, which integrated … WebT-REx™ Cell Lines For your convenience, Invitrogen has available three mammalian cells lines that stably express the Tet repressor. T-REx™-293 cells and T-REx™-HeLa cells express the Tet repressor from pcDNA™6/TR and should be maintained in medium containing blasticidin. T-REx™-U2OS cells express razzie and oscar same year

Flp-In System: For Generating Constitutive Expression Cell …

WebJan 1, 2012 · We described here a simple method to display full-length antibody on Flp-In 293 cells (F293) by transient transfection of a single vector into the cells. The full-length antibodies can easily be detected on cell surface by flow cytometry 36–48 h post-transfection. 2 Materials. WebThe protein expression level of the Q141K variant increased about two-fold when Flp-In-293 cells were treated with MG132. In contrast, the protein level of ABCG2 WT was little affected by the same treatment. After treatment with bafilomycin A1, the protein levels of ABCG2 WT and Q141K increased 5- and 2-fold in Flp-In-293 cells, respectively. sims 2 build buy cc