Web13 hours ago · GLUT1 is one of the key molecules responsible for glucose transport and initiating glucose uptake in cells, where it is also involved in the regulation of aerobic glycolysis [39., 40., 41.In CD4 + T cells, decreased expression of GLUT1 decreases the glycolysis level, thus inhibiting their activation [42, 43].TEPP-46 is an allosteric activator … Human umbilical vein endothelial cells (HUVECs) were purchased from Lonza (Basel, Switzerland). A ReverTra Ace RT Kit was bought from TOYOBO (Osaka, Japan). Anti-GLUT1 (#12939) and Alexa Fluor 488 anti-rabbit IgG antibodies were purchased from Cell Signaling Technology (Danvers, MA). Anti-GLUT3 (PA5-72331) … See more HUVECs were cultured in endothelial cell growth medium (EGM-2; Lonza, Basel, Switzerland) on fibronectin-coated culture dishes or plates (Corning, Schiphol, Netherlands). The cells were used between passages 3 … See more Total RNA was extracted from HUVECs using an RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol and reverse transcribed to cDNA with the use of a ReverTra Ace RT Kit (TOYOBO). Real … See more GLUT1 localization was determined by immunocytochemistry analysis based on a laboratory and antibody recommended protocol [14]. HUVECs were cultured on 13-mm poly-l-lysine-coated coverslips (Matsunami Glass, … See more Total protein lysates were prepared by collecting experimental HUVECs in 1 × sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) sample … See more
Expression of hypoxia-related glucose transporters GLUT1 …
WebJan 24, 2024 · Human macrophages infiltrating hypoxic regions alter their metabolism, because oxygen becomes limited. Increased glycolysis is one of the most common cellular adaptations to hypoxia and mostly is regulated via hypoxia-inducible factor (HIF) and RAC-alpha serine/threonine–protein kinase (Akt) signaling, which gets activated under … WebApr 12, 2016 · As Glut1 is upregulated on tumor cells in response to hypoxia, we assessed whether surface Glut1 levels regulate the antigen responsiveness of human T lymphocytes in both hypoxic and … inc長野 youtube
Hypoxia-inducible factor-1α is the therapeutic target of the SGLT2 ...
WebMar 23, 2001 · H-ras and Hypoxia Additively Increase glut1 mRNA Levels. To examine how oncogenic transformation by human H-rasaffects expression of the glut1gene, we stably … WebJun 18, 2024 · Recently, the structure and distribution pattern of GLUT1 has also drawn wide concern. Some studies have found that it is an inward-open uniporter with a single N-glycosylation site (10, 11), and some have showed a markedly punctate staining pattern of GLUT1 on cell membranes under deconvolution fluorescence microscopy ().However, … ind 101 excelsior