Mcherry dna配列
WebNEB 5-alpha Competent E. coli were transformed, and DNA was prepped (Qiagen Hilden, Germany) and sequence verified. EYFP (pMSCV-IRES-YFP II) was a generous gift from Mohammed Azam (CCHMC). EGFP (MIGR1; AddGene #27490), tdTomato-N1 (Addgene #54642), and pMSCV-mCherry (AddGene #52114) were obtained from AddGene. Cell … WebmCherry is a member of the mFruits family of monomeric red fluorescent proteins (mRFPs). As a RFP, mCherry was derived from DsRed of Discosoma sea anemones unlike green fluorescent proteins (GFPs) which are often derived from Aequorea victoria jellyfish. Fluorescent proteins are used to tag components in the cell, so they can be studied …
Mcherry dna配列
Did you know?
The gene for mCherry is 711bp long, and the protein is made up of 236 residues with a mass of 26.722 kDa. The crystal structure of mCherry was determined in 2006. It contains 3 alpha helices and 13 beta sheets which make up the beta barrel. The chromophore in mCherry is made up of three amino acids, methionine, tyrosine, and glycine, which are post-translationally modified into an imidazolinone. The number of these residues in sequence are 71, 72, and 73 respectively. The … WebpRetroQ-mCherry-C1 Vector Information; pRetroQ-mCherry-C1 vector sequence in GenBank format; 632568 : Z2568N: pRetroQ-mCherry-N1 Vector Retroviral Gene …
WebEGFP is a basic (constitutively fluorescent) green fluorescent protein published in 1996, derived from Aequorea victoria. It is reported to be a rapidly-maturing weak dimer with moderate acid sensitivity. Web1. MG1655 Organism: Escherichia coli str. K-12 substr. MG1655 (E. coli) Submitter: INSTITUT PASTEUR Date: 2024/09/17 Assembly type: Assembly level: Complete …
WebmCherry inserted into cloning site. Vector type Mammalian Expression Promoter CMV Selectable markers None Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 37°C Growth Strain (s) Top10/P3 Copy number High Copy Cloning Information Cloning method Restriction Enzyme 5′ sequencing primer Custom WebGene Name mCherry タイプ ORF タイプ Fluorescent reporter 詳細 Variant of mRFP1 generated by mutagenesis アプリケーションノート Commonly used red fluorescent …
WebPlasmid mCherry from Dr. Rob Parton's lab contains the insert mCherry and is published in J Cell Biol. 2024 Dec 6;220(12). pii: 212693. doi: 10.1083/jcb.202405065. Epub 2024 Oct … bosch wav28g40 saturnWebTry the full version of SnapGene for 30 days. One trial is allowed per computer. Your files will remain accessible with the free SnapGene Viewer. bosch wav28g40 homeprofessional smarteWebPlasmid pcDNA3.1-mCherry from Dr. David Bartel's lab is published in Cell. 2024 Jul 12;174(2):350-362.e17. doi: 10.1016/j.cell.2024.05.022. ... What is virus associated … bosch wav28g40 homeprofessionalhttp://www.cc.kochi-u.ac.jp/~tatataa/tech2024/gene/plasmids.html bosch wav28g40 homeprofessional kaufenWeb1 mrt. 2024 · We investigated whether transcription of H2B-mCherry in donor cells is required for the transfer of this reporter gene. The knockdown of H2B-mCherry using shRNA in MDA-MB-231 significantly blocks ... bosch wav28eh7nl home professionalWebThe pmR-mCherry vector can be transfected into mammalian cells using any standard transfection method. If desired, stable transfectants can be selected using G418. … hawaii coroner\u0027s officeWebPlasmid pcDNA3.1-mCherry from Dr. David Bartel's lab is published in Cell. 2024 Jul 12;174(2):350-362.e17. doi: 10.1016/j.cell.2024.05.022. ... What is virus associated DNA, and why do I have to order it? What virus titer will I receive? Viral storage; Antibody storage; View All Viral Preps and Antibodies articles; bosch wav28g40 homeprofessional waschmaschine