Phi 6 bacteriophage
WebDec 21, 2024 · But there are some star-shaped structures now under study in the lab that also give us plenty of reason for hope. One of them is a tiny virus called bacteriophage phi-6, which researchers are studying in an effort to combat a similar, but more-complex, group of viruses that can cause life-threatening dehydration in young children. Thanks to a ... WebNational Center for Biotechnology Information
Phi 6 bacteriophage
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Φ6 (Phi 6) is the best-studied bacteriophage of the virus family Cystoviridae. It infects Pseudomonas bacteria (typically plant-pathogenic P. syringae). It has a three-part, segmented, double-stranded RNA genome, totalling ~13.5 kb in length. Φ6 and its relatives have a lipid membrane around their nucleocapsid, a … See more The genome of Φ6 codes for 12 proteins. P1 is a major capsid protein which is responsible of forming the skeleton of the polymerase complex. In the interior of the shell formed by P1 is the P2 viral replicase and transcriptase … See more RNA-dependent RNA polymerases (RdRPs) are critical components in the life cycle of double-stranded RNA (dsRNA) viruses. However, it is not fully understood how these important enzymes function during viral replication. Expression and characterization of … See more • Double-stranded RNA viruses See more 1. Detailed molecular description 2. Descriptions of tests of evolutionary theory by the Turner Lab 3. Descriptions of tests of evolutionary theory by the Burch Lab See more Φ6 typically attaches to the Type IV pilus of P. syringae with its attachment protein, P3. It is thought that the cell then retracts its pilus, pulling the phage toward the bacterium. Fusion of the viral envelope with the bacterial outer membrane is facilitated by the phage … See more Φ6 has been studied as a model to understand how segmented RNA viruses package their genomes, its structure has been studied by scientists interested in lipid-containing … See more WebDec 9, 2016 · The 6 dsRNA bacteriophages were isolated from legume samples or lakes with Pseudomonas syringae as the host. Here, we report the first Pseudomonas aeruginosa phage phiYY with a three-segmented...
WebBacteriophages as a weapon against superbugs. Bacteriophages are viruses that target, infect, and destroy specific bacteria without harming human and animal cells. For this reason, they provide formidable weapons for preventing and treating bacterial infections. Since the discovery of bacteriophages in the early 1900s, researchers have explored ... WebPhage Φ6 is a segmented RNA virus involved by a phospholipid envelope (fatty) with spike proteins at its surface, with ~80–100 nm size, structural features similar to several human viruses, namely Influenza (belongs to Orthomyxoviridae family), SARS-CoV-1, SARS-CoV-2 and Middle East Respiratory Syndrome-associated coronavirus (MERS-CoV) (belong …
WebApr 10, 2024 · Raman spectroscopy offers the potential for fingerprinting biological molecules at ultra-low concentration and therefore has potential for the detection of viruses. Here we review various Raman techniques employed for the investigation of viruses. Different Raman techniques are discussed including conventional Raman spectroscopy, … WebAug 17, 2024 · A novel method of testing has been developed by Campden BRI which uses as a surrogate the bacteriophage Phi6. This phage is an ideal surrogate being an enveloped RNA virus very similar in size and structure to coronaviruses. ... with a measured reduction in infectivity of greater that 6.5 log. Share this Article. Subscribe To Our Newsletter ...
WebAgabian-Keshishian N, Shapiro L. Stalked bacteria: properties of deoxriybonucleic acid bacteriophage phiCbK. J Virol. 1970 Jun5(6):795-800 PubMed ID 4193836: Method: Electron microscopy: Comments: ... Bacteriophage phi 29 ID: 109734 Average cell radius. Bacteria Staphylococcus ...
WebFeb 21, 2024 · The phi-X174 bacteriophage was selected as the most appropriate surrogate because it satisfies all of these criteria. The phi-X174 bacteriophage is one of the smallest known viruses (25-27nm) and is nonenveloped, with icosahedral (nearly spherical) morphology. It has excellent environmental stability, is non-infectious to humans, and has … shelia flynnWebOct 14, 2024 · Abstract. Phage Phi6 is an enveloped virus considered a possible nonpathogenic surrogate for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and other viral pathogens in transmission studies. Larger input amounts of bacteriophage Phi6 are shown to delay and protect the phage from environmental decay, both when the … splichWebBacteriophage phi-12 ( Gottlieb et al. 2002a, b) belongs to the same genus ( Cystovirus) as bacteriophage phi-6, and they are considered to be closely related evolutionarily. Both phages have three segments of chromosomal double-stranded RNA. splice yarnWebDec 14, 2024 · Phi 6, an RNA pseudomonas phage, has been previously evaluated as a surrogate for multiple virus pathogens including ebolavirus ( 10 – 12) and various human respiratory viruses ( 13, 14 ), including coronaviruses such as SARS-CoV-2 ( 3, 15, 16) on surfaces ( 16 – 18 ). splice wiringWebDec 29, 2024 · Bacteriophage phi 6 has 80-100 nm diameters, comparable to SARS-CoV-2 (60-140 nm) (Zhu et al., 2024). The bacteriophage phi 6 is not contagious to humans and is used as an encouraging surrogate ... splicifyWebIn this study, we determined inactivation efficiencies of two coronaviruses (ssRNA) and an enveloped dsRNA bacteriophage surrogate in buffered aqueous solution (pH 7.4) using five commonly available UVC devices that uniquely emit light at different wavelengths spanning 222 nm emitting krypton chloride (KrCl*) excimers to 282 nm emitting UVC LEDs. shelia fontWebThe protein composition of the lipid-containing bacteriophage phi 6.Y was investigated and the synthesis of phage-specific proteins in the host bacterium Pseudomonas phaseolicola HB10Y was studied; the extent of viral protein synthesis fell off exponentially with increasing delay time between irradiation and infection. Expand shelia fields