Thp1 pma lps
WebDec 12, 2024 · Mutation of S401 in THP1 cells demonstrates that the p38 MAPK/TFEB pathway plays a particularly relevant role during monocyte differentiation into … WebNational Center for Biotechnology Information
Thp1 pma lps
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WebJan 5, 2014 · 结果：1．PMA刺激THP一1分化为巨噬细胞的过程中，LPS＋IFN—Y使iNOS、IL－6及TNF－Q表达升高（P＜0．05），IL一4则使iNOS、IL－6、TNF—a表达降低而IL－10表达水平明显升高（P＜O．05）；2．PMA的刺激使THP－1分化为巨噬细胞的过程中上调CDl37L的表达，1F1使THP一1在分化为巨 ... WebFeb 17, 2024 · The cell cycle was determined by Flow cytometry. G, LX2 cells were treated with LPS pre-exo, (50μg/ml) or un-exo, and then wound healing assays were performed. H, …
WebJan 5, 2024 · Challenging THP-1 cells with LPS: Different concentrations of LPS were added in cultured THP-1 cells in three replicates, and incubated for about 24 hours to stimulate … WebCell culture supernatant obtained from THP1 cells, transfected with pcDNA3.1-GAS5 and then exposed to LPS, PMA, and PMA + LPS as described above, was analyzed using …
WebJul 14, 2024 · UMB was added during the PMA-induced M0 differentiation phase, the polarization phase (LPS + IFN-γ or IL4 + IL13), and the polarized M1 or M2 cocultured with AGS cells. Figure 6 shows that UMB increases the M1/M2 ratio with the maximum ratio at the first phase, PMA, and with the minimum ratio at the last phase, cocultured. WebMar 5, 2009 · On the next day, in case of THP1 cells, they were treated with phorbol-12-myristate-13-acetate (PMA) for 24 hours, washed, and incubated for additional 24 hours …
WebTHP-1 cells were incubated with PMA to differentiate into THP-1 derived macrophages. Then 4 μg/ml OVA, 4 μg/ml MIC3 and 1 μg/ml LPS were added into the culture medium respectively. The levels of TNF-α were evaluated by ELISA. Data are expressed as the means ± S.D. of six independent samples for each group.
WebAug 7, 2008 · The THP-1 cells were exposed to PMA for 72 h and then treated with LPS with or without URG, RG, or both as described above. As expected, both URG and RG extracts … how many thai people speak englishWebThe THP-1 cells were differentiated into macrophages under the stimulation of phorbol 12-myristate 13-acetate (PMA) and then treated with LPS to build a cellular inflammation model. The cell viability was detected by CCK-8 assay. … how many thai restaurants in usaWebMar 30, 2024 · Similar findings were observed when other tumor cell lines, including the lung cancer cell line A549 and the glioma cell line U87MG, were co-cultured with PMA-primed … how many thana in dhaka districtWebAug 9, 2015 · We set up a model of macrophage polarization, starting from THP-1 monocytes differentiated into macrophages using PMA (Phorbol 12-myristate 13 … how many thai speak englishWebSince both IFN-γ and LPS levels are found to be elevated in obesity and shown to be involved in the regulation of metabolic ... THP-1 cells were treated with 10 ng/mL PMA (Phorbol 12 … how many thalamus are there in the brainWebNational Center for Biotechnology Information how many thai vowels are thereWebDuring the differentiation inducement by PMA for THP-1 cells, both CD14 and CD54 molecules were demonstrated to be up-regulated and TLR-2 to be Table 1. The physicochemical characteristics of JDBM extract. Samples pH Osmolality(mOsm/kg) Mg2+ concentration(mg/L) Ctr(1640 Medium) 7.5±0.17 283.2±1.5 10 10% Extract 7.53±0.2 … how many thalidomide babies were there